- Pds1 / Esp1 Mutants
- pds1∆
- pds1∆ in nocodazole
- bub2∆ pds1∆ in nocodazole
- mad2∆ pds1∆ in nocodazole
- cdc20∆ pds1∆
- cdc20∆ pds1∆ clb5∆
- CLB5-db∆ pds1∆
- CLB5-db∆ pds1∆ cdc20∆
- CLB1 clb2∆ pds1∆
- esp1-ts
- GAL-PDS1-db∆ esp1-ts
- GAL-PDS1-db∆
- PDS1-db∆
- GAL-ESP1 cdc20-ts
PDS1-db∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: kd2pds"=kd3pds"=0
Arrest: Exit mitosis at t=191.2 min with sister chromatids still attached.
Experiments:
Cohen-Fix, O., Peters, J.M., Kirschner, M.W. and Koshland, D. (1996). Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev. 10:3081-3093.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Inviable, Figs. 1A and 2B, sister chromatids do not separate.
Comments: Since Pds1 is never degraded, Esp1 is always inactive and the sister chromatids remain attached.
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